Sparteine exerts anticancer effect on human cervical cancer cells via induction of apoptosis, G0/G1 cell cycle arrest and inhibition of VEGFR2 signalling pathway

Purpose: To investigate the anticancer effects of sparteine against human cervical cancer.
 Methods: Cell viability was determined by CCK8 assay, while 4′, 6-diamidino-2-phenylindole (DAPI) staining used for determination apoptosis. cycle analysis done with flow cytometry, cell invasion monitored using Transwell assays. Protein expressions were Western blotting.
 Results: The results revealed that inhibited cancer cells halfmaximal inhibitory concentration (IC50) ranging from 10 to 25 µM. Sparteine exerted more profound antiproliferative on DoTc2 cells, IC50 However, minimal cytotoxicity observed in normal as evident 80 triggered generation ROS and apoptotic death cells. induction apoptosis accompanied upregulation Bax expression downregulation Bcl-2 expression. caused arrest at G0/G1 phase cycle, suppressed cyclin A B1. assay data showed decreased ability also phosphorylation VGFR2 a concentration-dependent manner.
 Conclusion: exhibits significant activity may prove beneficial chemotherapy.